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In this study, a novel plant expression system was used to create virus-like particles (VLPs) as novel vaccines for the H5 and H9 variants of Influenza A virus. These variants are important pathogens circulating in poultry farms, and present a potential source of spillover infection into humans. This study assessed the quality of the immune response to these VLPs using ELISA antibody binding assays and retroviral pseudotype models of infection.